王 焱,王竟夷,黄 亮,班立桐,李奕葶,党红慧,王 玉*
(天津农学院农学与资源环境学院,天津 300384)
摘要:本试验以双孢蘑菇As2796为研究对象,对双孢蘑菇液体菌种固态化工艺进行了研究。通过测定固态化菌种萌发时间、菌丝生长速度、菌丝长势、机械强度,确定优化后包埋剂组成为:海藻酸钠3.5%、硅胶4.0%、脱脂乳粉2.0%、谷氨酸钠4.0%、羧甲基淀粉钠2.0%,滴落于2.5% CaCl2溶液中凝固。在此条件下制备的固态化菌种,萌发时间34 h,菌丝生长速度为4.9 mm/d。出菇数据表明,固态化菌种23 d即可满瓶,产量达到14.72 kg/m2。
关键词:液体菌种固态化工艺;双孢蘑菇As2796;萌发时间;菌丝生长速度
中图分类号:S646.11 文献标识码:A 文章编号:1674-506X(2021)01-0024-0006
Preliminary Study on the Solid-state of Technology Liquid Spawn of Agaricus bisporus
WANG Yan, WANG Jing-yi, HUANG Liang, BAN Li-tong, LI Yi-ting, DANG Hong-hui, WANG Yu*
(College of Agronomy & Resources and Environment, Tianjin Agricultural University, Tianjin 300384, China)
Abstract:The solid-state technology of liquid spawn of Agaricus bisporus As2796 was studied in this study. According to the germination time, mycelial growth rate, mycelial growth potential and mechanical strength, the optimized embedding agent group was: sodium alginate 3.5%, silica gel 4.0%, skimmed milk powder 2.0%, sodium glutamate 4.0%, carboxymethyl starch sodium 2.0%, dropped into 2.5% CaCl2 solution for solidification. Under these conditions, the germination time of solid-state strains was 34 h, and the growth rate of mycelium was 4.9 mm/d. The data of mushroom production showed that the solid-state strain could be filled in 23 days, and the yield reached 14.72 kg/m2.
Keywords:the process of liquid spawn immobilized; Agaricus bisporus As2796; germination time; mycelial growth rate
doi:10.3969/j.issn.1674-506X.2021.01-004
收稿日期:2020-06-17
基金项目:天津市种业科技重大专项(16ZXZYNC00210),中央引导地方科技发展专项(20ZYCGSN00380),国家级大学生创新训练项目(201710061040),天津市蔬菜产业体系(ITTVRS2017015),天津市教委科研计划项目(2017KJ87),天津市特支计划-青年拔尖人才项目(TJTZJH-QNBJRC-2-12)。
作者简介:王焱(1996-),男,本科。研究方向:食用菌液体菌种。
*通信作者
引文格式:王焱,王竟夷,黄亮,等.双孢蘑菇液体菌种固态化工艺的初步研究[J].食品与发酵科技,2021,57(1):24-28,74.
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